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NuPAGE Bis-Tris 8, 10, 12, 412 1.5 to 300 kDa 12 months 3550 min Separation of small- to medium-sized proteins. Population genetic and biophysical evidences reveal that purifying selection shapes the genetic landscape of Plasmodium falciparum RH ligands in Chhattisgarh and West Bengal, India. Ideal for Western blot transfer and analysis, and all techniques in which protein integrity is crucial. Non-synonymous amino acid alterations in PfEBA-175 modulate the merozoite ligand's ability to interact with host's Glycophorin A receptor. Group 1 metabotropic glutamate receptors trigger glutamate-induced intracellular Ca2+ signals and nitric oxide release in human brain microvascular endothelial cells. Effects of chemotherapy on placental development and function using in vitro culture of human primary cytotrophoblasts. PGRMC1 phosphorylation affects cell shape, motility, glycolysis, mitochondrial form and function, and tumor growth. Publishing research using ab116028? Please let us know so that we can cite the reference in this datasheet.Īb116028 has been referenced in 16 publications. Storage: Stable for up to 3 months at 4☌. This product was previously called Prism Ultra Protein Ladder (10-245 kDa). An extra tidbit: After you have done your Western blot visualization, stain the blot with amido black: Stain: 0. Precision Plus Protein Unstained Recombinant Protein Standards are Strep-tagged, enabling immunodetection and molecular weight determination on western blots. Review other protein ladders in the unstained and prestained protein ladder guide. Use a pre-stained protein ladder to track transfer of proteins from a gel to a membrane. Unstained natural protein standards allow accurate MW determination with uniform band intensities on SDS-PAGE gels stained with Coomassie Blue or zinc. Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.Ready-to-use: Supplied in a loading buffer for direct loading on gels.The protein ladder is supplied in gel loading buffer and is ready to use. This prestained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer). Accurate protein sizing on SDS-polyacrylamide gels and Western blots.Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa.Proteins (0.02 to 0.05 mg/mL of each) in 62.5 mM Tris-H3PO4 (pH 7.5 at 25☌), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol.The PageRuler Unstained High Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use. In addition, the ladder proteins contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. The 150kDa band is of greater intensity and serves as a reference band. The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. Quality tested-each lot evaluated by SDS-PAGE and Western blotting.Tagged-each protein in the ladder contains an integral Strep-tag™ II Sequence.Reference band-150kDa band has greater intensity for easy orientation.Sharp bands-excellent accuracy for detection in with coomassie or silver stains.Ready-to-use-supplied in a loading buffer for direct loading on gels.Size range-8 proteins spanning 60 to 250kDa.Thermo Scientific PageRuler Unstained High Range Protein Ladder is a mixture of eight (8) unstained proteins (60 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.įeatures of PageRuler Unstained High Range Protein Ladder: